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Bootscanning

Bootscanning was first described by Mika Salminen:

Salminen MO, Carr JK, et al. Identification of breakpoints in intergenotypic recombinants of HIV type 1 by bootscanning. AIDS Res Hum Retroviruses. 1995 Nov;11(11):1423-5

The basic principle of bootscanning is that mosaicism is suggested when one observes high levels of phylogenetic relatedness between a query sequence and more than one reference sequence in different genomic regions.  Using default values for our demonstration data, we obtain:



When the bootscan is completed, we are notified that a number of groups appear to be potentially mosaic.  Clicking on each of these in the legend allows the user to view the bootscan plots for each of these as a query.  The notification regarding possible evidence of recombination, and the threshold for this notification, is available in Preferences under the Options menu.

Note: The raw values of the bootscan can be saved in comma-delimited format from the File menu.